The two oxygen levels simulate cellular oxygen conditions in either a tumor environment or normal cellular environment.
Cells were cultured under either normoxic (5%) or hyperoxic (19%) conditions grown in the presence of the Microdiscovery Discovery “Killer Plate” drug compound library under a series of time points (24, 48, or 72 hrs). We compared multiplex flow cytometer and single-read plate reader HTS platforms by screening drug compound libraries on Jurkat and Ramos cell lines.
The major limiting step when working with hematopoietic cells, however, is genetic manipulation and this is hindering important advancements. Research within immunotherapy and immuno-oncology is accelerating at an unprecedented rate as positive results from CAR-T clinical trials are encouraging new collaborations and large investments.